SEA-FISHERIES LABORATORY. 481 



Water Analyses — 



Number of intestinal bacteria per c.c. of surface 



water from : — 



Channel near Wyre Dock (23 February, 1911), 1, 0, 2, 0. Mean = 0-75. 

 Channel near Wardley's (10 March, 1911), 26, 24, 17, 24, 20. Mean = 22. 



Roosebeck and Barrow Channel Mussel Beds. 



27 June, 1912. 



Sample of mussels from the Scar. 



Five mussels emulsified in 250 c.c. of sterile water. 



Intestinal bacteria absent in l/10th mussel. 



[Note. — In this analysis MacConkey's bile-salt 

 broth was used. All tubes containing 

 l/10th and lesser fractions of a single 

 mussel were unchanged after 48 hours' 

 incubation. The " enteritidis-reaction " 

 also failed with l/50th part of a mussel.] 



1 November, 1912. 



Sample of mussels taken from fisherman's bag 

 immediately after the shell-fish had been taken. 



Ten mussels emulsified in 250 c.c. of sterile water. 



2 c.c. of emulsion = 



(0-08 mussel) contained 33 intestinal bacteria. 

 25 

 21 

 24 

 29 

 1 c.c. of emulsion = 



(0-04 mussel) contained 15 intestinal bacteria. 

 18 

 8 

 6 

 11 

 Mean No. of intestinal bacteria per mussel = 310. 



The "enteritidis-reaction" was obtained with 1/12 



but not with 1/ 120th mussel. 



No recent analyses of mussels from the Barrow 



Channel beds have been made. These are hardly 



necessary since the conditions are such that serious 



pollution cannot be avoided. 



FF 



