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TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



The number (1,321) at the beginning of the first day was 

 not actually observed, since the plate was so crowded as to 

 be impossible to count. This number has been extrapolated, 

 as will be discussed later on. 



At the beginning of the ninth day the plate (containing 

 70 organisms) was put aside and incubated for a day longer 

 in the cold. Ten colonies were then subcultured on nutrient 

 agar and re- cultivated in the same series of media. The same 

 reactions were given as in the case of the original colony. A few 

 drops of the dulcite-broth culture, after incubation for four 

 days, were added to sterilised sea-water as before, and the 

 number of bacteria contained in this liquid were again estimated 

 from day to day. The results obtained were : — 



Experiment II. 



Days. 



1 



2 



3 



4 



5 



No. of bacteria per 1/10000 c.c... 

 1/1000 c.c. ... 

 1/100 c.c 



21 

 231 



(2310) 



84 

 886 



4 



43 



36 



2 



The count (2,310) for 1/100 c.c. for the first day (that is, 

 at the beginning of the experiment) was not observed. It is 

 the count for 1/1000 c.c, 231, multiplied by 10. 



The plate counted on the third day (with 43 colonies) 

 was incubated for a day longer in the cold, and eight colonies 

 from it were subcultured on nutrient agar. These were again 

 subcultured in the various media, with again the same results 

 as were given by the original colony. One of the dulcite-broth 

 tubes was again taken, after incubation for four days, and 

 a few drops from it were added to half a litre of sterile sea- water 

 as before. The number of bacteria present was estimated 

 from day to day with the following results :— 



