15G TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



ascertaining the position of fat and glycogen in the tissues, 

 and tracing any changes which may occur. 



The following technique has been adopted : — 



Fat : (a) Tissue fixed in 4 % Formaline ; frozen 

 sections cut and stained with Sudan III ; 

 mounted in glycerine jelly. 

 (b) Fixative 10 % potassium bichromate and 

 glacial acetic ; paraffin sections stained 

 with Sudan III and Delafield's haema- 

 toxylin.* 



Glycogen : Fixative absolute alcohol ; wax sections 

 stained with LugoFs solution and mounted in 

 xvlol balsam. 



* See Bell, J. oj Pathology, No. 1, XIX, 1914 



