SEA-FISHERIES LABORATORY. 



345 



Thirteen of the colonies identified as belonging to the 

 colon group were then subcultured on nutrient agar in 

 sloped tubes, and after 48 hours' incubation these growths 

 were subcultured in bile-salt broth, glucose, lactose, 

 mannose, and cane sugar broths, and in litmus milk. 

 Again, after 48 hours' incubation the cultures were 

 examined. The results of these tertiary subcultures are 

 shown on the next table. It is obvious that the colonies 

 Nos. 6 and 14 are not those of B. coli. In two cases 

 (7 and 11) the bacilli were non-motile, and in three cases 

 (1, 6, 7,) the motility was very feeble. But at least six 

 out of the thirteen colonies examined in detail proved to 

 be those of typical B. coli. 



The white colonies present on the primary plates did 

 not belong to bacteria possessing any special significance. 



1st Analysis. 



Results of Tertiary Subcultures from Primary 

 Plates. 



Mussel 



Bile- 

 Salt. 



Glucose. 



Lactose. 



Mannose. 



Cane 

 Sugar. 



Milk. 



Motility. 



I . 



.. ag . 



.. ag 



.. ag . 



.. ag . 







.. a c 



... ? + 



2 . 



■• ag . 



•• ag 



.. ag . 



.. ag . 



■ ag 



a 



... + 



3 • 



.. ag . 



•• ag 



•• ag . 



■■ ag . 



. 



.. a c 



... + 



6 . 



.. ag . 



.. ag 



• • a g . 



.. ag . 



o 



.. a c 



... ? + 



6 . 



.. ag . 



.. ag 



o 



•• ag . 







o 



... + 



7 • 



.. ag . 



•• ag 



.. ag . 



.. ag . 



o 



.. a c 



... + 



7 • 



•• ag . 



.. ag 



.. ag . 



.. ag . 



• ag 



.. a c 



— 



7 • 



.. ag . 



.. ag . 



•• ag . 



.. ag .. 







.. a c 



... ? + 



7 • 



.. ag . 



.. ag 



.. ag . 



.. ag . 



. 



.. a c 



... + 



9 • 



.. ag . 



.. ag . 



.. ag . 



.. ag . 



. d 



.. a c 



... + 



ii 



.. ag . 



.. ag . 



•• ag . 



.. ag . 



o 



.. a c 



— 



H ■ 



a 



a 



a 



a 



o 



.. a c 



... + 



2 3 • 



.. ag . 



.. ag . 



•• ag . 



.. ag . 



• ag . 



.. a c 



... + 



Explanation of Symbols : — 

 acid ; g = gas ; c = clot ; d = discolouration. 



