346 



TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY 



The second analysis was conducted in precisely the 

 same manner. A sample of about 20 mussels was 

 collected from Great-out and Little-out Skears, one mussel 

 being taken from each part of the skears visited. This 

 sample was therefore a thoroughly representative one. 

 One drag was then made in the middle of Ring-Hole, and 

 a similar quantity was taken from the sample. Five 

 mussels from each of these samples were examined as 

 before. In the case of the Ring-Hole shellfish the 

 enteritidis reaction was not tried for. The results of 

 these primary inoculations are shown on the next table. 

 Distinctive white colonies were absent in each case, and 

 the numbers of colon-like colonies vary from 20 down- 

 wards. In the case of two of the Outer Skear mussels the 

 enteritidis reaction was atypical. 



2nd Analysis. 



Primary Subcultures on Neutral-red, Bile-salt, 

 Lactose Agar Plates. 



Locality. 

 Ring Hole " 



Out 



Skc 



ssel. 



" Colon-like" 

 Colonies. 



White 

 Colonies. 



Enteri- 

 tidis 

 re-action. 



I 



. .. about 20 









2 



.. „ 20 .. 









3 



14 









4 



9 









5 



7 

 some patches 









6 



4 







.. not typical 



7 

 8 



7 

 16 





 



+ 

 .. not typical 



9 



7 

 some patches 







+ 



IO 



14 







+ 



