348 transactions liverpool biological society. 



Tertiary Subcultures from Broth Inoculated with 

 1 cc. Water. 



Locality. q ij." Glucose. Lactose. Mannose. q Milk. Motility. 



"Out" ... ag ... ag ... ag ... ag ... o ... ac ... — 

 Skear 



"Baiting ... ag ... ag ... ag ... ag ... d ... ac ... + 

 Knot " 



It was not possible at the time to make an exhaustive 

 analysis of the sea-water in the vicinity of the skears, but 

 several samples were collected and examined. Sterilised 

 glass-stoppered bottles were filled with water from off 

 Great-out Skear, from just over the main sewer outfall, 

 from near Baiting Knot, and from Ring-Hole. In the 

 cases of the outer skears, and Baiting Knot, only one 

 culture was made from each sample. One cc. of the water 

 was added to bile-salt broth and the latter was incubated. 

 A reaction was then obtained and plates were made from 

 the primary culture. Colonies supposed to be those of 

 B. coli were then selected, and examined in pure sub- 

 culture. In the case of the Baiting Knot sample the 

 colony selected was B. coli, but in the case of the colony 

 from the outer skear sample motility was not observed. 

 The microbes present were therefore not typical Bacillus 

 coli. 



In the case of the water sample from near the main 

 sewer outfall, and from Ring-Hole, a number of dilutions 

 were made, each rb-th less concentrated than the one 

 immediately above. One cc. of the original or diluted 

 liquid was put into a sterile Petri dish and neutral red 

 agar at a temperature of 40°C. was poured in and allowed 

 to solidify. After 48 hours' incubation the plates were 

 counted. A dozen plates were made. Only primary 

 inoculations were made. In the case of the water from 



