44 
doubtless due to the bottle of broth from which it was inoculated 
having been infected by some other bacterium. Cultivations were 
from gelatine, in a ten ounce flask of agar-agar, gave also what appears 
to be the characteristic smell. 
From the above experiments it is reasonable to suppose that the 
virus, as received, was alive and active, as it was capable of starting 
w 
EXPERIMENTS ON RATS. 
one teaspoonful of salt. hree other rats were fed on rice which had 
been steeped in broth culture diluted with salt water, in the propor- 
tion of one bottle, of 900 c.c., of broth to nine litres of water and 
nine teaspoons of salt. 
'These rats were each put in a separate cage and fed on sugar-cane 
and boiled rice. Up to the 27th September, the date of my leaving 
the estate, they remained in perfect health. The incubation period, 
given in the letter accompanying the virus, is fourteen days. 
The virus was fed to other rats on the 13th and 14th and several 
as also fed 
were used, and none of them showed any ill effects from the treat- 
ment. 
Two rats were given a subcutaneous injection, by means of a 
hypodermic syringe, of a mixture of sterilized water and about 15 
per cent. of the gelatine culture, taken from two tubes. The rats 
were chloroformed and the injection made near the root of the tail, 
one receiving two minims and the other four minims of the fluid. 
The result of this experiment was also negative, as it had no effect 
whatever on the rats, either locally or constitutionally. 
APPLICATION IN THE FIELD. 
From the 12th to the 25th September, infected food was laid in 
the fields. Bread, rice and Chinese green peas were used, soaked in 
both the broth and gelatine virus, diluted as directed. About 25 
