206 PROFESSOR RUTHERFORD ON THE 
HAIN, and others, it was nevertheless deemed desirable to definitely ascertain 
whether or not, in the case of the liver, this antagonism also obtains ; accord- 
ingly, four-fifths of a grain of atropia sulphate, dissolved in 3 cc. of water, was 
injected into the duodenum at a. The effect being somewhat doubtful, three- 
fifths of a grain dissolved in 3 ce. of water was injected into the jugular vein. 
The bile-secretion speedily fell, and it is evident from the chart that within 
half-an-hour after the administration of the second dose the effect of the physo- 
stigma had entirely disappeared. 
Necropsy.—There was decided irritation of the duodenal mucous membrane 
to the extent of 8 inches below the pylorus. Evidence of only slight purgative 
action was found in the small intestine. 
Experiment 534. Dog that had fasted eighteen hours. Weight 13°6 kilo- 
| Experiment 534. 
Secretion 
Secretion of bile per 
of bile per | kilogramme 
1b”. of dog : per 
hour. 
cc. 
0°35 
0°45 ; 
0°50 lo 138 cc. 
) 
2°70 0'758 ce. 
s 
0°65 
0°50 : 
0°15 ho 121 ce. 
Fig. 584.—Secretion of bile before and after Calabar bean, atropia, and lead 1°25 
acetate. 2 grains extract of Calabar bean with 1 cc. of bile and 5 ce. of 1°10 
water injected into the duodenum atc; 14 grain extract, with same, at c’; 
2 grains extract, with same, at c’; ¢ths of a grain of atropia sulphate in 4 ce, 
of water injected into the jugular vein at a; ths of a grain at a’; 8 grains 0°25 
of lead acetate in 20 cc. of water injected into the duodenum at J. 0-00 
grammes (fig. 534).—Two grains of the extract of Calabar bean, triturated 
with 1 cc. bile and 5 ce. water, were injected into the duodenum at c. The 
7 
