G, CARR ROBINSON AND W. L.. GOODWIN ON 
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Action of strong Nitric Acid on these Bases. 
When these: bases are dissolved in dilute hydrochloric acid, and the solution 
is boiled with the addition of a few drops of dilute nitric acid, the only 
apparent effect the latter has is that of a purifying nature, by causing the 
separation of a small quantity of a black tarry substance. This was observed 
by GREVILLE WILLIAMS, and was mentioned by him as a means of. purifying 
Cryptidine.* ) 
When the bases are treated with strong nitric acid an intensely purple 
solution is obtained, and if this solution be evaporated on the water-bath a 
sticky resinous mass remains. The resinous mass, when digested with water, 
is dissolved only to a very small extent, yielding a solution which, when treated 
with dilute hydrochloric acid and platinum chloride, gives a platinum salt 
showing percentage of platinum agreeing with that requiredt for a nitro- 
substitution body. Further examination into that portion of the resinous mass 
insoluble in water has led us to regard it as the nitrate of the base. A quantity 
of tetracoline was dissolved in strong nitric acid, and the solution evaporated 
on the water-bath; the resinous mass was digested with three successive 
quantities of water, and the insoluble portion dried at 100° C. A. brittle 
amber-coloured resinous substance, readily soluble in alcohol, was obtained. 
Analysis gave the following :— 
I. 0226 grms. gave— 
0509 ,, CO, = 61°41 per cent. carbon. 
0:12 » HOH 589 " hydrogen. 
II. 0:2105 ,, gave— 
04755 ,, CO, = 61°60 per cent. carbon. 
O12" = Ol or89 .. hydrogen. 
These analyses would show the resin to be the nitrate of tetracoline, 
C,,H,,;N.HNO,, which requires— 
Calculated ek een oP 
Carbon, : ; 61°54 61°41 61°60 
Hydrogen, ; ’ 598 5'89 5'89 
Nitrogen, ; : 11°96 
In the first paper on this research t the extreme difficulty experienced in 
* “Transactions R.S.E.,” vol. xxi. p. 401. : 
+ “Some New Bases of the Leucoline Series,” part ii., Transactions R.S.E., vol. xxix. part i. 
t “ Transactions R.S.E.,” vol. xxviii. part ii., “ On some New Bases of the Leucoline Series,” 
