266 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 
6and7p.m. Each gathering represents therefore roughly 
about 12 hours fishing, or more exactly, the day gathering 
extended over 10 hours, and the night one over 14 hours. 
The contents of each of the four nets was examined and 
entered in the note-book separately, but in a few cases, 
when there seemed no object in keeping them apart, two 
or more of the gatherings taken in the same locality and 
period of time, were preserved in the same bottle. Hach 
day and each night, then, of the voyage is represented in 
the collection by one or more—sometimes four or five— 
separate bottlesful of plankton. 
‘““T may state at once that I saw no marked difference 
between the day and the night gatherings. It must be 
remembered, in this connection, that the source of the 
water was not quite close to the surface, but at least 
14 ft. down.” 
It was in much the same method as the above that the 
two collections now described were made. 
Captain Wyse’s voyage extended from Delagoa Bay 
direct to Calcutta, leaving Africa on April 30th, 1898, and 
reaching Calcutta on May 14th. Thence, after six weeks’ 
stay, on June 28th he steamed direct for Aden, through 
the Red Sea and Suez Canal and along the Mediterranean 
to Gibraltar. 
Mr. G. W. Herdman travelled by a coasting steamer up 
the south-eastern African coast, starting from Durban on 
May 7th, 1899, and calling at intermediate ports. Thence, 
he followed Capt. Wyse’s route through the Red Sea and 
Suez Canal into the Mediterranean, leaving Port Said on 
June 17th. 
The two routes and the positions of the stations are 
shown upon the map (Pl. XV.) which illustrates this paper. 
