72 TKANSACTIONS LIVEKPOOL BIOLOGICAL SOCIETY. 



The present report deals almost exclusively with the 

 Bacteriology of the oyster and the behaviour of the 

 Bacillus typhosus in sea-water and in the body of the 

 oyster. The subject of the green colouration in oysters 

 is also discussed but will be treated more fully in a 

 subsequent report. The questions investigated are the 

 following : — 



I. The indemnification and differentiation of Bacillus 

 typhosus and B. coli communis. 

 The action of sea-water upon the growth of B. 



typhosus. 

 The Bacteria present in the alimentary canal of 



the oyster. 

 The infection of the oyster with B. typhosus, and 



its removal by washing. 

 The green colouration and green disease in oysters. 



II. 



III. 



IV. 



V. 



I. — The Identification and Differentiation of Bacillus 



typhosus and B. coli communis. 

 We have systematically tested the majority of the chief 

 differential reactions upon samples of Bacillus typhosus 

 and B. coli obtained from numerous sources, and have in 

 all cases found unmistakable differences between the two 

 bacilli. 



Table showing Differences of Beaction. 



Source 



Fermentation. 

 Glucose Gelatine 



Indol 



Reaction 



Coagula- 

 tion 



Potassium Iodide 

 Potato Gelatine 



A. B. typhosus. 







1. Institut Pasteur 



2. From spleen of 

 typhoid patient 



3. Prof. Detepine 



4. Prof. Wright 

 (Netley). 



none 

 j j 



> j 

 j? 



none 



55 

 ) > 



slight trace 



none 



j i 



55 



clot slow- 

 ly formed 



very small growth 



5 1 

 1 t 



, 6. j-Dr.S.'Woodhead 





none 

 > » 



none 



55 

 » J 



8 ) 



p* [ Dr. Kanthack 



10. Institute of pre- 

 ventiveMedicine 



> j 



55 



> > 



55 



J ) 

 5) 

 J> 

 51 



