74 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



II. — The Action of Sea-water upon the Growth of the 

 B. typhosus. 



Experiment 



I. 





Experiment IV. 











No. of 





No. of 









Bacilli 





Bacilli 



At time of mixing 



. 



- 



29,250 



At time of mixing - 



130 



After 21 hours 



. 



- 



20,475 



After 6 hours 



41 



,5 45 „ 



- 



- 



9,945 



u 23 „ - - 



31 



„ 71 „ 



- 



- 



9,360 



„ 48 „ - - 



38 



„ 93 „ 



. 



. 



5,850 



„ 72 „ - - 



negative 



.. 271 „ 



- 



- 



260 



„ 247 „ 



1 



„ 340 „ 



- 



- 



11 



„ 316 ,, 







Experiment 



II. 





Experiment V. 





At time of mixing 



. - 



. 



1,300 



At time of mixing - 



31,200 



After 21 hours 



. 



. 



1,105 



After 172 hours 



9,360 



„ 45 „ 



. 



. 



780 



„ 244 „ - - 



325 



,, 71 ,, 



. 



. 



650 







,, 95 ., 



. 



. 



325 



Experiment VI. 





„ 271 „ 



- 



- 



2 



At time of mixing - 



325 



„ 340 „ 



- 



- 







After 172 hours 



2 



Experiment 



III. 





Experiment VII. 





At time of mixing 



. 



. 



22,750 



At time of mixing - 



325 



After 5 hours 



. 



. 



17,550 



After 504 hours (water kept 





,, 23 ., 



. 



. 



11.700 



at 8° C. to 10° C.) 



79 



., 48 „ 

 ,, 72 „ 



- 



; 



3,250 

 3,260 



Experiment VIII. 





n 247 „ 



- 



- 



455 



At time of mixing - 



325 



„ 316 „ 



- 



- 



325 



After 504 hours 







These results are fairly uniform. When a large number 

 of Bacilli are added to the water their presence may be 

 demonstrated longer than in cases where small quantities 

 are used. Fourteen days would appear to be the average 

 duration in sea-water incubated at 35°C, whilst when kept 

 in the cold their presence was demonstrated on the twenty- 

 first day.* There appears to be no initial or subsequent 

 multiplication of the Bacilli. Between 40 and 70 hours 

 after infection there is less decrease than at other periods ; 

 but there is no evidence of increase in numbers of the 

 Bacilli when grown in sea-water either when incubated 

 or at ordinary temperatures. We do not think, however, 



* Dr. Cartwright Wood, however, finds that the typhoid Bacilli may 

 possibly persist for two months, 



