78 TEANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



In the following series of experiments infected oysters 

 were taken, the duplicates of which as seen in the pre- 

 ceding table contained comparatively large numbers of 

 the B. typhosus, and were subjected to a running stream 

 of pure sea-water. The result is definite and uniform, 

 there is a great diminution or total disappearance of the 

 B. typhosus in from one to seven days. 



Table showing Organisms present after Washing. 











No. of 





Oyster 



Inoculated 



Washed 



Examined 



Colonies 



Kind of Organisms present 



Agar 



9 



1 



Aug. 25 



Aug. 26 



Aug. 30 



80 



2 colonies B. typhosus 



2 



>j 



Aug. 28 



J 5 



23 



B. typhosus present 



3 



Aug. 26 



5? 



5) 



44 



>5 55 



4 



,, 



Aug. 29 



) : 



40 



5 5 55 



5 



Aug. 27 



>j 



,, 



5 



1") 55 



6 



jj 



, i 



Aug. 31 



700 



abundant B. typhosus 



7 



Aug. 28 



Aug. 30 



55 



55 



B. typhosus present 



8 



Aug. 26 



Aug. 28 



Sept. 3 



4 



? B. typhosus 



9 



Aug. 27 



Aug. 29 



! 5 



10 



no B. typhosus found 



10 



>> 



5) 



55 



8 



3 colonies of B. typhosus 



11 



Aug. 28 



Aug. 30 



Sept. 4 



4 



1 colony of B. typhosus 



12 



55 



Sept. 3 



5? 



200 



majority B. typhosus 



13 



Aug. 31 



? 



5 5 



4 





14 



Aug. 28 



Sept. 3 



Sept. 6 



65 



no B. typhosus, but Proteus 



15 



Aug. 31 



55 



5 5 



5 



1 B. typhosus 



16 



»j 



Sept. 5 



,, 



70 



half of colonies B. typhosus 



17 



>j 



Sept. 3 



Sept. 10 



1 



no B. typhosus 



18 



5? 



Sept. 5 



Sept. 11 



2 



? B. typhosus 



V. — The Green Colouration and " Green Disease " 

 in Oysters. 



We have continued our investigation of green oysters 

 from various localities, including both the healthy green 

 oysters grown at Marennes and other places on the West 

 and North coasts of France, and in the Koach Kiver in 

 Essex, and also what we regard as the unhealthy oysters 

 which show a pale greenness due to a leucocytosis. The 

 green patches visible to the eye on the mantle of these 

 oysters correspond to accumulations of the leucocytes 



