SEA-FISHERIES LABORATORY. 



251 



RESULTS OF THE PRIMARY CULTURES ON NEUTRAL-RED 

 AGAR PLATES. 



Mussel. 



Number of Colon-like Colonies. 



1 





82 



2 





7 



3 





200 



4 



■ 



46 



5 





120 



6 





146 



7 



Very 



numerous, colonies run together. 



8 





200 



9 





196 



10 





200 



Anaerobic cultures for the demonstration of B. 

 enteritidis sporogenes were also made from the 10 mussels 

 examined above. All these fish, except No. 4, gave the 

 typical enteritidis reaction. In No. 4 the milk was 

 clotted, but the peculiar torn condition of the clot was 

 not produced. 



Nine of the colon-like colonies on the primary 

 neutral-red plates were inoculated on agar to obtain pure 

 sub-cultures, and the latter were then tested in detail. 

 Four of these colonies gave all the reactions for B. coli, 

 so that nearly half of the colonies counted were those of 

 this latter bacillus. 



The method of analysis recommended by Dr. A. C. 



Houston* was also utilized in this case. One mussel was 



extracted, with all precautions to ensure sterility of the 



vessels, apparatus, &c, and was beaten up with 100 cc. of 



-2nd Report Roy. Comm. Sewage Disposal, p. 135 [cd. 1178], 1902. 



