262 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



Plates 1 and 2 contained B. coli. 



Various white colonies were also studied in pure sub- 

 culture, but none was identified. A bacillus was isolated 

 from plate 2 which produced simple acidity in bile-salt, 

 glucose broth, and in glucose broth, no reaction with 

 lactose, mannose, sucrose, or glycerine, and acidity and 

 clotting in milk. Mr. Lewis, of the Pathological Depart- 

 ment in the University of Liverpool, informed me that 

 those reactions are identical with those produced by a 

 bacillus which he isolated from one of the South African 

 War infected Army blankets, examined some time ago. 

 This, therefore, is most probably also a microbe of 

 intestinal origin. 



All the anaerobic milk cultures made gave the typical 

 enteritidis reaction. 



It was concluded then that the mussels from both 

 Crook Skear and Bazil Point were infected with sewage 

 matters. This conclusion is thoroughly borne out by 

 Mr. Scott's and my own examination of the conditions of 

 the beds, and by the study of the bacteria adhering to the 

 paper picked up from the sands. At the same time 

 comparison with the results obtained in the analysis of the 

 Egremont mussels shows that the degree of pollution of 

 the Lune mussel beds is not excessive. 



IY. Deep-Sea Oysters. 



During the summer of 1904 Professor ITerdman sug- 

 gested that a bacteriological examination of oysters and 

 other shellfish than mussels, from sources which might 

 reasonably be regarded as free from sewage pollution, 

 should be made in order to afford a comparison with the 

 results obtained from mussels taken from inshore areas. 

 Accordingly I collected and examined several samples of 

 oysters from various otf-shore grounds. The first analysis 





