SEA-FISHERIES LABORATORY. 263 



of this kind that was made was one of the stomach 

 contents of shrimps from the Blackpool closed ground. 

 On 11th June, 1904, while trawling there I took back 

 half-a-dozen shrimps to the laboratory. The stomachs 

 and livers of these animals were dissected out with sterile 

 scissors and forceps and put into a small beaker and mixed 

 with 6 cc. of sterile water. Various quantities of this 

 emulsion were then inoculated in neutral-red agar liquid 

 at 42° C. This was poured into Petri dishes and allowed 

 to set and then incubated. The results were : — 



1 



Number of 

 " Colon-like " Colonies. 



2 cc. of emulsion (== 

 1 „ „ ( = 



o-i „ „ (= 

 o-oi „ „ (= 



1 shrimp) ' 



i .. ) 



Ath „ ) 



1*0*1" .. ) 



1 surface, 4 deep. 

 1 „ 3 „ 



1 deep. 



Sterile. 



Six of these colonies, ascribed to B. coli (or its 

 congeners) were then studied in pure sub-culture. In 

 no case were reactions typical of this organism obtained. 

 All the reactions obtained were such as might be ascribed 

 to staphylococci, or organisms of ubiquitous distribution. 

 The results of this analysis were, therefore, ambiguous, 

 and no opportunity has yet been found to repeat it. 



Deep-sea Oysters from near Liverpool 

 N. W. Lightship. 



On July 6th, 1904, a special visit was. made in the 

 "John Fell" to the grounds in the vicinity of the Liver- 

 pool N.W. Lightship in order to get a sample of oysters 

 for analysis. This ground was selected because oysters 



