SEA-FISH EK I ES LA JMltATORY. 



265 



growing, were then sub-cultnred on a nutrient agar and 

 examined in detail. The results were: — 





Motility. 



Bile salt 

 broth. 



Glucose 

 broth. 



Lactose 

 broth. 



Mannose 

 broth. 



Sucrose 

 broth. 



Glycerine 

 broth. 



Milk. 



ony 

 [ 



ony 



+ 



Acid and 



very slight 



gas. 



Acid, gas. 



Acid, gas. 

 Acid, gas. 



0. 



Acid, gas. 



Acid, gas. 

 Acid, gas. 



Acid, gas. 

 Acid, gas. 



O 



(after 6 

 days). 



Acid, gas 

 (in 24 hrs). 



Acid. 

 Acid, clot 



The broth and milk cultures were examined after 24 

 hours, but the glycerine cultures after 6 days. 



Neither of these organisms is B. coli. Colony 4 is 

 most probably that of B. acidi lactici, and colony 2 is 

 possibly that of B. cloacm. McConkey concludes that 

 this latter microbe is only an attenuated form of B. coli. 



Three oysters were tested for the enteritidis reaction : 



Oyster 2 

 4 



Enteritidis reaction. 



Oysters from near Morecambe Bay 



Lightship. 



On July 21st, 1904, a visit was paid to this ground for 

 the purpose of getting oysters for analysis. A trawl net 

 was used, and was shot 1 mile S. by W. of the vessel and 

 dragged to the East. Over three dozen oysters were taken, 

 and a dozen were brought back to Liverpool the same 

 evening and stored as before. This ground is far removed 

 from sewage contamination ; it is about 16 miles from the 



