266 



TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY 



nearest land, and is almost in the fairway of the Channel. 

 The depth was 13 fathoms, and the bottom muddy. No 

 land debris w T as taken in the net. 



Six oysters were examined early on the following day — 

 two of these had the gills choked np with a large mass of 

 " black-spat " larvae. One had vivid green patches over 

 the region of the viscera and heart. 



A larger quantity than usual of the stomach contents 

 (0*3 to 0*5 cc.) was removed for inoculation. Six neutral- 

 red agar plates were made and incubated for 48 hours. 

 At the end of this period the plates were sterile. 



B. coli (and other sewage) organisms were therefore, 

 absent. 



A similar quantity of stomach contents was inoculated 

 in milk tubes, and incubated anserobically. The results 

 were : — 





Enteritidis reaction. 



Oyster 1 







„ 2 



— , milk discoloured, but not clotted. 



,. 3 



+ 



» 4 



— 



» 5 



— , milk discoloured, but not clotted. 



On September 22nd, 1904, another visit was made in 

 the " John Fell " to Morecambe Bay lightship. The trawl 

 was shot about 5 miles South of the ship. About a score 

 of large oysters were obtained, and from these six were 

 taken at random and put apart for analysis. They were 

 allowed to stand on deck for about 18 hours before being 

 landed at Piel. They were then analysed (at the Piel 

 laboratory) as before. 



