338 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 



RESULTS OF PRIMARY CULTURES ON NEUTRAL-RED AGAR. 



Mussel. Number of "Colon-like" Colonies. 



Mussels 

 from near 

 the break - 



3 



i 



About 98 

 



in the 

 sewer pipe. 



4 



y 5 



76 with several diffuse patches. 

 60 



Mussels 

 from below 

 the break - 



8 



1 

 

 One diffuse patch. 



in the 

 sewer pipe. 



9 

 10 



1 with a diffuse patch. 

 1 



Primary cultures were also made from the stomach 

 contents in litmus milk under anaerobic conditions with 

 the following results: — 



PRIMARY ANAEROBIC CULTURES IN LITMUS MILK. 



Mussel. 



Enteritidis reaction. 



Mussel. 



Enteritidis reaction. 



1 



+ 



6 



+ 



2 



+ 



7 



No reaction. 



3 



No reaction. 



8 



+ 



4 



+ 



9 



+ 



5 



+ 



10 



+ 



The results of the tertiary cultures on the various 

 sugar media showed that Bacillus coli communis was 

 present in mussels 2, 4, 9 and 10, and probably in all the 



