690 Variations in a Copepod Crustacean. [September, 
distinguishable into regions, with two pairs of antenne, the ante- 
rior pair much larger, either one or both of the latter often trans- 
formed into an auxiliary, prehensile, copulative organ. They 
possess neither a carapace nor a bivalve shell; but have three 
pairs of mouth-parts and five pairs of swimming feet. Females 
with external egg-sac. 
Family of Calanide—Body elongate, similar to that of Pon- 
tellidze, anterior antennz very long, usually of twenty-four to 
twenty-five joints. In the male sex the right, rarely the left an- 
tenna is transformed into a geniculating, prehensile organ. Pos- 
terior antennz comparatively large, two-branched. Mandibulary 
palpus two-branched, similar to the posterior antenne. . Maxille 
with a large, manifold-lobed palpus. Maxillipeds powerfully devel- 
oped. Fifth pair of legs large, either similar to 
the four preceding or alike in both sexes, or ab- 
errant from those and dissimilar in the sexes; :n 
the male a clasping organ to assist, together with 
the right antenna, in copulation. Heart present. 
Eyes highly developed, median and mobile. Male 
genital glands unpaired, female glands paired. 
Single median egg-sac of orbicular shape. 
We now drop some live specimens into alco- 
hol; they will die in a few seconds; leaving these 
crimson colored little Crustaceans in alcohol for 
some time, they will soon become pale and finally 
yellowish, transparent. We now pour off the 
alcohol and add a strong solution of pure car- 
mine in concentrated ammonia and a little gly 
erine, macerating them for about one day. Then 
we wash the staining liquid gradually off, first 
with water and then with alcohol, and preserve 
them in glycerine for examination. 
Placing a male specimen (Fig. 4) on a glass 
slide under the dissecting microscope with low 
power, we now proceed with the dissecting 
needles to separate successively the different 
pendages, viz., the anterior long antenn&, the 
he ih a les with 
teridr  “antenas as shorter posterior antennz, the mandibles 
ale. 
palpus, the superior maxillipeds, the maxillz, the 
inferior maxillipeds, the four pairs of natatory legs, the fifth, trans-__ 
