676 The American Naturalist. [July, 
CARPOPHYTA. 
ASCOMYCETEE. 
PyRENOMYCETEZ.—(Vols. I., IL, and Add.)—Perisporiacez, 481; 
Spheriacex, 5448; Coryneliaceze, 2; Hypocreacex, 640; Dothidi- 
aces, 351 ; Microthyriacez, 65 ; Lophiostomacee, 213; Hysteriacee, 
372; Hermihysteriacez, 3. Total, 7575 species. 
HypopERME#.—(Vol. VII.)—Uredinez, 1224 ; Ustilagineze, 284. 
Total, 1508 species. 
Phymetospheriacee, 16; Onygenacee, 6; Laboulbeniacezm, 15. 
Total, 37 species. (Vol. VIII.) 
SPH#ROPSIDEZ.—(Vol. III.) —Spherioidacez, 3690 ; Nectrioidacez, 
44; Leptostromacex, 203; Excipulacee, 143. Total, 4080 species. 
Melanconiacez, 606 species, (Vol. III.). 
HyPpHomYcETE2.—(Vol. IV.)—Mucedinacee, 1147; Dematiacez, 
1579; Stilbacex, 344; aace, 594. Total, 3664 species. 
, 30 species. (Vol. VIII.) 
DiscomycETE&.—(Vol. VIII. )—Caliciacex, 78; Gymnoascacez, 
51; Cordieritacez, 5; Patellariacex, 161; Phacidiaces, 268; Stic- 
tacez, 229; Bulgariacee, 152; Dermateacex, 255; Ascobolacee, 
130; Pezizacez, 1948; Heloellacex, 169; Cyttariacee, 7. Total, 
3453 Species. 
TUBEROIDE&.—(Vol. VIIL)— Elaphomycetacez, 21; Cenoccoca- 
cee,1; Tuberacee, 102; Endogonacez, 6. Total, 130 species. 
BASIDIMYCETEE. 
GASTEROMYCETEZ.—(Vol. VII.)—Hymenogastracex, 78; Lyco- 
perdacez, 426; Nidulariacez, 61; Phallacez, 81. Total, 646 species. 
HYMENOMYCETE.E,—(Vols. V. and VI.)—Tremellacex, 258; Clava- 
riacex, 371; Thelephoracex, 884; Hydnacex, 427; Polyporacee, 
1972; Agaricacez, 4639. Total, 8551 species. 
There are thus 1100 species of Protophytes; 371 of Zygophytes; 
176 of Oöphytes, and 30,280 of Carpophytes. Of the latter again 
there are 12,703 Ascomycetex ; 9,197 Basidiomycetex, with 8,380 
probably, but not certainly, imperfect stages of the former. 
CHARLES E. BESSEY. 
The Preparation of Vegetable Tissues for Sectioning on 
the Microtome.—Vegetable tissues vary so much as to the amount 
of protoplasm, cellulose, and other substances contained, that the 
