722 The American Naturalist. [September, 
forty-eight hours, and developed an acid reaction in neutral 
nutrient solutions within a few days. The composition of these 
solutions is not stated, nor whether the growth of the organism 
is self-limited by the production of the acid. This production 
of acid serves to strengthen the belief that the bacillus is really 
the cause of the beet disease. The nature of the acid was not 
determined. 
(c) Production of Alkali.—Not recorded. 
(d) Formation of Pigment.—Brownish color on beets. 
(e) Development of Odors.—No odor. 
(f£) Enzymes.—Not determined. Cell walls are dissolved. 
(g) Other Products.—Not stated. 
(11) Effect of Dessication.—No statement. - 
(12) Thermal Relations : 
(a) Maximum for Growth—Not determined. If the bloat- 
ing of the cattle were due to this organism, it must be able to 
grow at blood heat, and the accurate determination of its 
thermal relations should not have been omitted. 
(b) Optimum for Growth Not determined. 
(c) Minimum for Growth—Not determined. 
(a) Death Point.—Not determined. 
(13) Relation to Light.—No statement. 
(14) Vitality on Various Media.—No statement. 
(15) Effect on Growth of Reaction of Medium (acid, neutral, . 
alkaline) —No statement. 
(16) Sensitiveness to Antiseptics and Germicides—No statement. 
(17) Other Host Plants —No statement. 
(18) Effect upon Animals.—No cattle could be had for experi- 
mental purposes; but the germ was carefully tested on rabbits 
and white mice, and was not pathogenic either when fed to 
them in carrots, rubbed into subcutaneous wounds, or injected 
into the blood by means of a Pravaz syringe. 
(III) Economic ASPECTS : 
(1) Losses.—Serious. 
(2) Natural Methods of Infection —Not known. 
(3) Conditions Favoring the Spread of the Disease—Not known. 
(4) Methods of Prevention No experiments, and no observa- 
tions. Disease not studied in the field. 
