802 The American Naturalist. [October, 
cayed mass; and from the leaves, before the disease has reached 
the bulb, it is impossible to get a sufficient quantity of the 
slime. 
On March 27, 1884, small quantities of yellow slime were 
taken from some slightly affected bulbs (double red Temple 
of Apollo) and inserted into wounds made for this purpose in 
the top of the leaves of different varieties. These were exam- 
ined daily for signs of disease which first appeared, in most 
cases, only aftera month. Distinct symptoms were apparent 
but unfavorable circumstances caused the loss of the leaves 
before the downward stripe had progressed very far. These 
plants stood in pots in the open air and were watered regu- 
larly, but the spring was very dry. Although in this case a 
month elapsed before external symptoms appeared, it is not to 
be inferred that so long a time always intervenes. On the 
other hand it is likely that in natural infections even a longer 
time may elapse before symptoms appear, since countless 
numbers of the bacteria are used in artificial infections, while 
natural infections are probably brought about in most cases 
by the entrance of a few bacteria which would require more 
time to produce visible results. On Oct. 27, 1884, the small 
unfolding leaves of each of anumber of sound hyacinth bulbs 
were wounded with a steel pen and some of the bacterial slime 
inserted into these punctures. The bulbs were then potted, 
kept in a place free from frost and examined from time to time. 
On Jan. 13, 1885, one plant showed the disease very distinctly. 
Two of the three infected leaves had stripes extending down- 
ward from the wound, each about 13 mm. Here, also, a long 
time intervened between the inoculation and the appearance 
of external symptoms. 
Another series of infection experiments was begun Dec. 28, 
1885, and completed in the spring of 1886. These plants were 
also inoculated with bacterial slime taken directly from dis- 
eased plants. These experiments were made on five plants of 
as many varieties, grown in carafes. All were kept in a cool 
place until Feb. 13, when they were transferred to a room reg- 
ularly warmed. The manner of inoculation and the results 
obtained are here summarized: La Tour d’ Auvergne (double 
