1895.] The Bacterial Diseases of Plants : 919 
time the whole mass was liquefied, and the bacteria were found 
at the bottom of the tube as a thin whitish layer. The liquid 
is then a clear brown, darker than the original gelatin. The 
-contents is almost odorless. © 
“ This experiment was repeated very often, and always gave 
the same result, only in subsequent experiments, it happened 
sometimes that the white globules did not appear. This, how- 
ever, is not surprising, since I then employed a mixture (glu- 
cose, extract of meat, gelatin) of slightly different composition, 
and since, moreover, the temperature was not always the same. 
‘On peut naturellement infecter aussi quelques tubes au moyen 
-de Bactéries prises dans d’autres tubes; cela n’a jamais rien 
-changé aux résultats.” 
No gelatin roll or plate cultures were made, and the be- 
havior of the organism in stab and streak cultures is not care- 
fully described. 
(8) Agar.—No account of any experiments on agar media. 
(4) Potato, etc—Nothing mentioned. 
_ (5) Animal Fluids.—The first artificial medium was made by 
adding a little meat extract and grape sugar to a decoction of 
meat which had been kept for some time in spirits, and was 
freed from the latter by washing and boiling in distilled water. 
It was then boiled for an hour in an additional quantity of 
-distilled water and the sugar and meat extract added. It 
remained clear for ten days, was then reboiled, cooled quickly, 
and a small quantity of the yellow slime introduced, the 
greatest care being used throughout to avoid contaminations. 
The second day this fluid became distinctly clouded, and this 
-clouding increased for four days, and then remained the same. 
The inoculations that failed were from this culture. The slime 
‘used to infect this culture came from a single vascular bundle 
of a freshly cut bulb. It was scraped off on a flamed cover- 
glass, which was then thrown into the fluid. The organism 
also grew well in a solution of meat extract to which glucose 
had been added. This was the fluid culture medium ordinarily 
employed, and there is no mention of any other. The exact 
composition of the medium is not given. 
