(No. 9). This vine was 215 centimeters long and bore a 

 number of small leaves and 17 large ones, 10 of which aver- 

 aged 20 cm. in breadth. March 21, 2:30 p. m. The stem near 

 the earth was cut under water and put at once into 1 per cent, 

 eosine water. 1 2:43 p. m. The stain is now distinct in all of 

 the principal veins of a leaf only 15 cm. from the end of the 

 stem, i. e., it has passed up the stem a distance of two meters 

 in less than 13 minutes, probably in 10 to 12 minutes. 2:47 

 p. m. The red stain is now distinct in the veins of the small 

 undeveloped uppermost leaves of the stem. 3:25 p. m. Slight 

 droop of the foliage, but much less than in No. 10 (a similar 

 vine in 10 per cent, eosine water). Foliage decidedly less red 

 than that of No. 10. 4:35 p. m. Leaves drooping very decid- 

 edly. The leaves of No. 10 are flabbier and redder, but much 

 less fluid has passed up the stem. 5:10 p. m. About 21 cc. 

 of the eosine water has passed up the stem in 2 hours and 40 

 minutes. March 22, noon. Leaves, tendrils and surface of the 

 young fruits reddish. The stain does not make its way readily 

 into the coiled tips of the tendrils. Many of the leaves are 

 dry shriveled, so that they crackle on touch. Stem not shriv- 

 eled. Most of the petioles are still turgid and but little stain is 

 visible in them, except in a few toward the top of the vine. 

 4:00 p. m. Not nearly so red as No. 10. Stem quite green and 

 not noticeably shriveled. The stem of No. 10 in the 10 per 

 cent, eosine has shriveled decidedly to-day. March 23, 12:25 

 p. m. About 10 cc. of the stain has passed up the stem since 

 last night. 4:30 p. m. About 10 cc. of the stain has gone up 

 the stem since the last record. March 25, 12:30 p. m. About 

 20 cc. of the stain has passed up the stem since the last record. 

 Most of the leaves are crisp dry, but the terminal ones are still 

 moist, although shriveled and soft like old rags, the parenchyma 

 being yellow and the veins bright red. Most of the petioles 

 are bright red, and all of them are limp and hang straight 

 down ; the stem has shriveled and become reddish, except the 



1 Kstilled water containing Dr. Grubler's " Eosine Soluble in water." 



