1038 The American Naturalist. [December, 
period the albumen appeared considerably corroded and easily 
crumbled upon slight pressure between the fingers. 
The substance was filtered and the filtrate then neutralized 
with dilute hydrochloric acid, upon which a slight precipitate 
of albuminate appeared. This was separated by filtration and 
the filtrate boiled, when a considerable coagulum of native 
albumen occurred. The native albumen was filtered off and 
the fluid then evaporated to about 300 cc. 
To this concentrated fluid strong alcohol was added in con- 
siderable excess upon which a copious fine floculent precipi- 
tate of albumoses and peptones appeared which slowly settled 
to the bottom of the beaker. This precipitate was collected 
upon a filter and dissolved in 600-700 cc. of water. The pre- 
cipitate completely dissolved, yielding an opalescent fluid 
which gave a strong xanthoproteic reaction and also a charac- 
teristic biuret test tending more strongly towards the rose-pink 
of a peptone. Ammonium sulphate was added to this solu- 
tion in the form of crystals till the whole was completely sat- 
urated, and after standing for several hours a copious precip- 
itate of albumoses gathered. This precipitate of albumoses 
was separated by filtration and the fluid was then treated with 
barium carbonate and barium hydroxide to precipitate the 
sulphate from the solution. After repeated treatments all the 
sulphate was finally precipitated and removed by filtration 
and the fluid was then boiled to a small bulk. About three 
volumes of strong alcohol were added and a fine floculent pre- 
cipitate appeared which settled on standing. This precipitate 
‘was collected and dissolved in water, and the solution then 
gave the various characteristic reactions for peptones. 
The first alcoholic filtrate was evaporated to a very small 
bulk, filtered and allowed to cool. After standing some time 
crystals of leucine and tyrosin were found upon microscopic 
examination, while the fluid also gave Hoffman’s test for ty- 
“rosin. 
At another time a set of parallel experiments was carried on 
in which equal amounts, by weight, of egg albumen were al- 
lowed to digest for an equal time with the same amounts of 
water extract of the glands, but under different circumstances. 
