SEA-FISHERIES LABORATORY. 137 



is formed by loose clusters of spores in artificial chambers 

 bounded by delicate strands of connective-tissue (cham.). 

 Outside, again, there are several concentric and partially 

 imbricate layers of connective-tissue (con. tis.). The 

 comparative looseness of the peripheral layers is partly 

 due to the shrinking together of the central residual mass 

 on fixation, and also to the fact that, once the " cyst " is 

 in the appendage, any further layer-formation, and, of 

 course, the proliferating infiltration, is free to expand. 

 From Hagenmiiller's account it is quite evident that his 

 cysts were nothing more or less than a similar modification 

 of the infiltrated condition. 



Before passing on to consider the spores, there is an 

 interesting point which a comparison of the two types of 

 infection, as set forth above, leads me to regard as being 

 very probable, namely, the potential independence of the 

 pansporoblasts in Glugea (i.e. their capability of existence 

 as separate individuals in certain circumstances). 

 Stempell (6), p. 263, has already suggested that 

 Thelohania, Pleistophora, &c. (those forms where the 

 whole individual becomes one reproductive organella), 

 are examples of a phylogenetic individualization of the 

 pansporoblasts. jN~ow, I am inclined to think this may 

 take place normally — for instance, in the condition of 

 diffuse infiltration — as a stage in the life cycle. Quite 

 probably " multiplicative reproduction " is, here, simply 

 a separation of the pansporoblast rudiments, as daughter- 

 individuals. Indeed, the whole nature of the diffuse in- 

 filtration in Glugea seems to me to support this idea. 

 There is no question of the individual parasites attaining 

 size, still less of any continuity of a protoplasmic mass 

 ramifying in and between the host's tissue-cells. It is 

 far rather a cell-infection, visible, when ripe, as sepa- 

 rate clumps of spores, each formed from, and 



