COMPARATIVE HISTOLOGY AND PHYSIOLOGY OF THE SPLEEN. 303 



antiseptically, and, except in one case, healed by first intention. After each operation, 

 two pints, or more, of milk were given immediately, and as much water as the animal 

 desired. 



Experiment I. — Part I. 



Day of 

 Experiment. 



Specific 

 Gravity. 



Per Cent. 

 Hb. 



Red Corpuscles. 



Leucocytes. 



Nucleated 

 Red Cells. 



Weight 

 in Kilo. 



1 



1057 



72 



6,590,000 



11,000 



None. 



6-5 



3 



1058-3 



72 



6,640,000 



12,000 



?> 





5 



1059 



72 



6,680,000 



12,000 



3J 



6 ; 6 



7 





Blood 



abstracted, 2*4 p 



er cent, of b 



ody weight. 





Four hours later. 



1049 



60 



4,880,000 



36,000 



None. 



6-47 



8 



1053 



60 



5,840,000 



34,000 



>) 



)) 



9 



1054-5 



60 



4,330,000 



15,000 



)> 





10 



1054-2 



62 



5,210,000 



16,000 



s> 





12 



1053 



58 



5,640,000 



20,000 



>> 





14 



1053-3 





5,135,000 



16,000 



)) 



6-47 



16 



1053-3 



54 



5,080,000 



18,000 



)! 





19 



1055-7 



54 



4,987,000 



20,000 



>> 



6-5 



20 





Bloo 



d abstracted, 3 pe 



r cent, of bo 



dy -weight. 





21 



1050 



38 



3,560,000 



32,000 



Very few (4J. 



6-5 



22 



1050 



36 



3,057,000 



32,000 



Few (30). 



... 



23 



1050 



38 



3,720,000 



28,000 



Few (20). 





24 



1050 



38 



3,900,000 



26,000 



Very few (5). 





26 



1051 



40 



3,760,000 





None. 



6-7 



28 



1050 



40 



3,960,000 



26,000 



j) 



... 



30 



1051-2 



40 



4,040,000 



16,000 



>> 





Note. — The figures in the column " nucleated red cells " indicate the average number of cells seen in single films 

 {\ means one in four films). 



There were no poikilocytes observed at any time ; but on the 10th day, three days 

 subsequent to the first bleeding, and afterwards, the red corpuscles varied in size slightly, 

 a few being 5, 6, or 7 p. 



On the 2lst day, the day following the second bleeding, the red blood-corpuscles 

 showed a slight tendency to invagination ; and nucleated red cells were seen for the 

 first time during the experiment, one or two being found after careful search through 

 several films. 



On the 22nd day, in a plain preparation of blood, there were seen several large cellular 

 bodies, one much larger than the others, which seemed to be giant cells. The largest cell 

 was much lobed, large buds projecting from its surface in all directions, giving an 

 appearance like that described in the giant cell of the spleen of the mouse, when 

 examined fresh in methyl salt solution. A small round cell was in contact with the 

 large cell, but apparently detached from it. The peripheral part of the protoplasm of the 

 large cell was tinted yellow here and there, as also was the whole of the protoplasm of 

 the small cell, and the latter seemed to contain a round nucleus. As the blood- 

 corpuscles ran together, other large cells were revealed, lying in spaces comparatively free 



