304 



DR A. J. WHITING ON THE 



from red cells. One special cell, somewhat smaller than the average, did not possess any 

 buds, but the nuclei were visible grouped in a heap in the middle of the cell, and were 

 surrounded by a comparatively narrow band of hyaline protoplasm which reached to 

 the periphery, and had a deep yellow colour. The fresh preparations showed a very 

 rich felt work of fibrin and very many blood plates, mostly in clusters. The films 

 contained several nucleated red cells, perhaps about thirty in each. Their protoplasm 

 stained more deeply with methyl blue than that of the majority of the non-nucleated 

 forms. Some of the latter, however, as Mum has recorded, show a similarly deep 

 staining, with an irregular surface as if of softer consistence than the majority, 

 suggesting that they had recently been in the nucleated condition. 



On the 23rd day several large cells were seen in a plain preparation of blood. The 

 nucleated red cells were not so numerous as on the preceding day, perhaps about twenty 

 in a film. Blood plates were very numerous. 



On the 2Uh day one large special cell was seen in one of two plain preparations 

 of fresh blood. There was a marked reduction in the number of nucleated red cells ; 

 about five were seen in each film. The red blood-corpuscles varied much in size. 



On the 26th day there were no large cells seen in several plain preparations of 

 blood. The nucleated red cells had apparently also disappeared ; but the films contained 

 many darkly stained softer red corpuscles. 



Between the second bleeding and the third an interval of forty-two days was 

 allowed to elapse. 



Experiment I. — Part II. 



Day of 



Experiment. 



Specific 

 Gravity. 



Per Cent. 

 Hb. 



Red Corpuscles. 



Leucocytes. 



Nucleated 

 Red Cells. 



Weight 

 in Kilo. 



1 

 2 

 3 

 6 



1052-2 



1052-2 

 1052-2 



54 



54 



54 



5,740,000 

 5,840,000 

 5,893,000 

 5,866,000 



18,000 

 14,000 

 16,000 

 14,000 



None. 

 None. 



6-6 

 6-6 



7 





Bloo 



d abstracted, 3 pe 



r cent, of bo 



dy weight. 





8 



9 



10 



1050-8 

 1050-5 

 The 



38 



40 



dog was k 



4,046,000 



4,120,000 



illed with chlorofo 



28,000 

 11,000 

 rm. 



None. 



6-4 



Note. — Day 1 of part 2 corresponds with day 56 of part 1. 



On the three days before the last bleeding, large special cells were found in the blood, 

 on an average one in a preparation. 



On the 8th day, the first day after the bleeding, in six plain preparations (four of 

 which were examined on Schafer's warm stage) there were seen many large cells, about 

 thirty or forty in each. Some were found in the hamiocytonieter preparation : one was 

 seen to occupy about the half of a ruled square. This had a blue-stained central portion 



