:50G DR A. J. WHITING ON THE 



were examined on the warm stage. Erythro blasts and nucleated red cells in fairly 

 large numbers and a few giant cells were recognised in all the fresh preparations, and 

 also in films of the pulp fixed in saturated solution of corrosive sublimate and subse- 

 quently stained. The giant cells in the fresh preparations were hyaline in appearance 

 and much budded, like those of the blood ; but amoeboid movement was not observed 

 in any of them on the warm stage. 



Sections of the Spleen showed comparatively few larger giant cells, perhaps about 

 sixty in each ; and they do not exhibit the appearances characteristic of active change. 

 There are smaller giant cells present in considerable numbers ; they are as a rule nearly 

 round, and have a small and but little lobed nuclear heap. The larger giant cells have 

 an unusually distinct perinuclear space. A giant cell and a few coarsely granular 

 protoplasmic corpuscles are occasionally seen in the follicles. There is a very small 

 number of erythroblasts and of nucleated red cells, but they are apparently almost, if 

 not quite, as numerous in j)roportion to the number of giant cells as in the other spleens. 

 There was seen in the veins a very small number of nucleated red cells. A few 

 pigment-holding cells were found in the follicles, and numerous pigment masses in the 

 pulp. 



TJie Bone-Marrow as seen in fresh preparations contained many erythroblasts, 

 nucleated red cells, and giant cells. The marrow of the ribs contained proportionately 

 more of these cells than that of the femur, indeed it seemed to be composed entirely of 

 them, and could be readily squeezed out from the cavity of the rib as a thin fluid. The 

 giant cells appeared to be less hyaline than those of the blood and spleen, and to have 

 fewer buds. Amoeboid movement on the warm stage was not seen. Sections of the 

 bone-marrow of the femur show numerous giant cells, on a rough estimate about 

 150 in each section. The yellow colour of their protoplasm is nearly as deep as that 

 of the red blood-corpuscles. A few grains of pigment are scattered here and there. 

 Very many erythroblasts and nucleated red cells are present, far more in proportion 

 to the number of giant cells than in any spleen I have examined. 



In a Lymphatic Gland taken from the neck there are a few erythroblasts and 

 nucleated red cells which appear to be colourless. There are a few somewhat large 

 multinucleated protoplasmic cells, like small giant cells, perhaps one or two in a section. 

 In the sinuses are large numbers of coarsely granular, uninucleated, protoplasmic 

 corpuscles that stain somewhat deeply with eosine ; their average size is about 10 m. 

 There are numerous pigment-holding cells present. 



In a Mesenteric Lymphatic Gland there arc erythroblasts in small number. In the 

 sinuses there are in addition numerous uninucleated vacuolated cells like those 

 characteristic of the spleen of the child ; and there are cells without vacuoles but 

 otherwise similar. 



Films of the blood taken from the splenic vein while the dog was dying did not 

 contain any nucleated red cells. 



