COMPARATIVE HISTOLOGY AND PHYSIOLOGY OF THE SPLEEN. 



307 



Experiment II 



Day of 



Experiment. 



Specific 

 Gravity. 



Per Cent. 

 Hb. 



Bed Corpuscles. 



Leucocytes. 



Nucleated 

 Red Cells. 



Weight 

 in Kilo. 



1 



1059-5 



84 



6,670,000 



7,000 



None. 



8-6 



2 



1059 



84 



6,740,000 



8,000 



)! 





3 



1058-5 



84 



6,780,000 



6,000 



)) 



8 : 6 



4 





Blood 



abstracted, 2-4 p 



er cent, of b 



ody weight. 





Three hours later. 



1054-5 



72 



5,613,000 



30,000 



None. 



• 8-4 



5 



1055 



72 



5,960,000 



10,000 



j) 



... 



7 



1055 



70 



6,250,000 



20,000 



; j 



... 



9 



10545 



72 



6,300,000 



20,000 



)j 



8-2 



11 



1057 



72 



6,140,000 



15,000 



)) 



... 



14 



1057 



74 



6,413,000 



12,000 



)) 



8-4 



15 





Blood 



abstracted, 3-2 p 



er cent, of b 



ody weight. 





16 



1052-2 



62 



5,020,000 



28,000 



None. 



8-4 



17 



1051-2 



56 



5,400,000 



30,000 



Few (10). 





18 



1053 



60 



5,440,000 



14,000 



Few (20). 





19 



1053-3 



58 



4,950,000 



14,000 



Very few (5). 



... 



21 



1054-5 



58 



5,090,000 



12,000 



None. 



8-2 



22 





Bloo 



d abstracted, 3 pe 



r cent, of bo 



dy weight. 





23 



1050-5 



48 



4,160,000 



54,000 



Few (10). 



8-2 



24 



1051-2 



48 



3,640,000 



86,000 



Numerous (40). 





25 



1051-2 



48 



4,030,000 



30,000 



Few (15). 





26 



1051-2 



48 



4,067,000 



16,000 



Very few (3). 



8-3 



28 



1051-2 



48 



4,060,000 



8,000 



Very few (2). 





29 



The 



dog was k 



illed with chlorofo 



rm. 







Poikilocytosis was never seen during this experiment, neither any tendency to 

 invagination of the red blood-corpuscles ; and there was very little variation in the size 

 of the red blood-corpuscles at any time. 



On the 17 th day, two days after the second bleeding, nucleated red cells were found 

 in the films for the first time, about ten in each. There were many more deeply 

 stained, non-nucleated red corpuscles with an irregular surface. 



On the 18th day several large cells were seen in a plain preparation of blood. Their 

 coarsely granular protoplasm had a faintly yellow tint. A somewhat small one (24 /* 

 in diameter) was noticed to have a rounded bud (8 n*) projecting from its surface, the 

 protoplasm of which was markedly yellow. Nucleated red cells were in larger numbers 

 than on the previous day, about twenty in a film. The blood plates were very numerous, 

 and the plain preparations showed a rich fibrin network. 



On the 19th day there were no large cells found in the blood, and only a few 

 nucleated red cells in the films, about five in each. 



On the 21st day there were no large cells found and no nucleated red cells, but 

 there were many softer, more deeply stained red blood- corpuscles. 



On the 23rd day, the first day after the third haemorrhage, large cells were not found 

 in six plain preparations of blood. A remarkable phenomenon was the very large 



