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made here of the cultures of Plagiochila asplenoid.es leaves. 

 Green-house specimens showing every appearance of vigor 

 were used and the cultures were kept for over two months, 

 but although the leaves remained green and vigorous, no sign 

 of any bud or rhizoid production was observed. This was 

 one of the species which Schostakowitsch grew successfully, 

 and it is apparent from there results that there are conditions 

 of the plant, when althougly apparenth vigorous, the power 

 of regeneration may be suppressed. 



The defoliated stems of Bryum produced some protonema 

 direct from the region of the leaf axil, but in the case of 

 specimens grown in the dark no distinct protonema growths 

 were noted. The abundance of production was much less 

 than in the case of Funaria hygrometrica. The paraphyses 

 here also were able to grow out into rhizo-protonema, by 

 the continued growth of the distal cell. This occurred however 

 only when they remained in connection with the stem, all 

 attempts at cultivating the detached paraphyses being to no 

 avail. The stems produced rhizoids quite abundantly , both 

 in ligth and darkness , and the production was not confined 

 to any particular portion of the stem. From the rhizoids 

 an abundance of buds was formed as lateral branches, and 

 in a light intensity which was not sufficient to produce 

 vigorous protonema. New shoots were produced by the 

 stems as lateral branches the same as in Funaria. These 

 appeared without any distinct localization of the point of 

 origin, Coming now from near the tip and now near the 

 base of the stem. The production of protonema was due 

 mostly to defoliation of he stem, since only in rare cases 

 was a protonema production noted from the whole plants 

 which were kept in the same conditions as the defoliated 

 stems. Rhizoid production was quite abundant from the 

 whole plants, but the growth in general was more abundant 

 from the defoliated stems. The production of new shoots 

 was not called forth by the defoliation of the stems, but was 

 only accelerated thereby, since whole plants also formed 

 lateral axillary branches, a mode of growth which is often 



