PHYSIOLOGY AND MORPHOLOGY OF CALIFORNIA HEPATICZ 4I 
The spores in the unbalanced culture solution germinated 
within six days after those in the normal Knop’s solution, which 
would indicate the existence of some stimulating factor in that 
solution. Another lot of distilled water was then prepared by 
allowing the first fourth or fifth part to distil as waste water, thus 
getting rid of the larger part of the volatile substances. Only 
the middle three fifths was saved and this was redistilled in the 
same manner. The best portion of this was again distilled and 
only the middle portion of the product was collected ; in this way 
water of a high degree of purity was obtained. This was used 
in the preparation of another distilled water culture and spores 
of Fossombronza sown eighteen days ago show no evidence of 
germinating. Spores sown on Knop’s solution, normal, germi- 
nated within fourteen days producing vigorous germ tubes with 
rather more than the usual amount of chlorophyll. These young 
plants have grown more rapidly and vigorously than those in 
distilled water that germinated approximately at the same time. 
Spores sown in the normal solution germinated two days later 
than those in either of the reduced solutions but in the former 
there was a far greater number of young plants two days after 
the first observation of germination. 
Spores of /imbriarza californica collected in March, 1905, 
were sown in Knop’s solution (normal) and germinated promptly 
within twelve days. Young plants in this culture that are now 
sixteen to twenty days old are characterized by the unusual 
length of the germ-tube, in some instances measuring more than 
one and a half millimeters from the basal end to the first cell 
cut off at the distal end of the germ-tube. In some instances 
two germ-tubes were seen to emerge from a single spore as 
figured by Campbell’ for Zargionia hypophylla. Spores of 
the same species were sown in an earthen saucer filled more 
than half-full of finely pulverized non-sterilized soil. These 
germinated promptly and in great numbers. Both cultures 
were so placed as to receive light from a north window: the 
Knop’s solution culture received somewhat the greater amount 
of light being in a glass dish, coated part way up with paraffin. 
None of the young plants of the soil culture produced germ- 
"Eo. cit., Ist €dz,) p..65% 2d ed-, p. 66. 
