SEA-FISHERIES LABORATORY. 207 
(>) from the seaward side of the same gutter, and as near 
as possible to No. 2 Black Buoy in Formby Deep. The 
sand was very clean and covered here and there with a 
scum of diatoms. ‘There is no source of pollution nearer 
than the mouths of the River Alt. The projected Crosby 
sewer outfall was not at the time discharging, and had 
not, in fact, been compteted. 
Formby: RESULTS oF PRIMARY CULTURES. 
Dilution. | No. of Red Colonies. Enteritidis Reaction. 
0g | 12 (50) Typical 
Ges 2 | 2 (sterile) Discolouration and clot 
(3 _ Sterile (sterile) No change [only 
ae | 45 (45) Typical 
Beye! 2 2G) Not typical 
ie _ Sterile (sterile) Not typical 
\ 
The figures in brackets give the results of the 
duplicate analyses. Duplicate enteritidis cultures were 
not made, 
Formpy: Resuuts or TERTIARY SUB-CULTURES. 
a | | i 
: P a OR uersey walt pieeetme 1 i, rs 
u Borate s Ue ea ieee lie Mean ie 
aL 2 = S| a IS OS =e ee = 
MS ie) — =) a aly 4 <4 — al => 
| | 
| | 
1 ag ag hos ACO all MEAL) 0) 0) ac ) 
2 ag ag MG | ES ) 0) ac 0 
3 ag ag UC Ole 0) 0) ) ac 0 
4 ag ag ag OE 40 9) 0 ac 0 
5 ag ag ag 0) ) 0) oy) ae 0 
6 ag ag ag 0) 0) OPN) 0 ac 0) 
7 ag ag ag 0) 0) a 0) a 0) 
8 a ag ag ag 0) a a ac 0) 
9 a a a a 0 a a | oO 0 
10 ag ag ag Co) ag oy) 0 ac ) 
In this and other tables a = acid formation; g = 
eas formation; d= discolouration; c = clot; o = no 
reaction. V.& P.=Voges’ and Proskauer’s reaction. 
