216 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 
fishing resulted from the prevalent distress in Barrow 
due to unemployment, and the mussels were sold in that 
district. The results of the analyses may be recorded 
here. 
First sampling, 50th March, 1908. The shell-fish 
were collected from the scar ground well down the beach. 
The methods of analysis were similar to those to be 
described in the case of the Conway experiment. 
+ 
PreL SHoreE, 30TH Marcu, 1908: Resutts or PRIMARY 
CULTURES. 
Mussel. No. of Red Colonies. No. of White Colonies. 
it 30. 20) 
2 85 + numerous patches About 60 
3 Numerous and fused Numerous and fused 
{ 63 -+ several patches 34 + several patches 
5 88 Several streaks 
6 50 + one large patch 43 + several! patches 
a 156 13 
8 22 | a 
9 135 + several patches = | 6 
10 Numerous and fused Numerous and fused 
A number of red colonies were examined in pure sub- 
culture, and comparative bacterial counts, using ordinary 
nutrient agar, were also made. ‘These results need not, 
however, be quoted. But it should be remarked that the 
above analysis indicates a considerable degree of pollution. 
The relatively large numbers of white (or translucent) 
colonies should be noted, for it 1s probable that excess of 
these colonies, growing on Griinbaum’s neutral-red agar 
medium, is indicative of recent, and presumably 
potentially dangerous pollution, 
