394 TRANSACTIONS LIVERPOOL BIOLOGICAL SOCIETY. 
up, each containing a definite amount of the extract, 
together with the substance to be acted upon. The 
“liver” extract was neutral, perhaps, if anything, slightly 
acid in reaction to litmus. The following were the 
various experiments made :— : 
(1) Bec. HCl + 4cee. dist. H,O + 3ec. extr. + ‘Sgr. fibrin. 
(2) dec. 0 Na,CO, + 4ee. dist. H,O + Sec. extr. + ‘Sgr. fibrin. 
(3) 9ec. dist. H,O + 3ce. extract + ‘Sgr. fibrin. 
+ 
N 
(4) DCC. 4H Na,CO, + 4ec. dist. H,O + 8ec. extr., allowed to 
stand three hours, and made acid. 
N 
(5) dec. _ HCl + 4ec. 2% peptone + 4 ce. extract. 
N 
(6) dee. 10 Na,CO, + 4cc. 4% peptone + 4ce. extract. 
(7) bee. H,O + 4cc. 2% peptone + 4ce. extract. 
(8) 5cec. 1% Methyl Acetate Sol. + 2cc. extract. 
(9) dee. 1% Methyl Acetate Sol. + dist. H,O (as control). 
To each of these two drops of IICN were added as a 
preservative. 
(10) Extract + Starch Solution ; toluol as preservative. 
After allowing the various mixtures to stand for 
forty-eight hours, the following tests were made : — 
The residues from 1, 2, 3, 4 and 7 were divided into 
three parts respectively, and the following products of 
digestion sought for—peptone or albumoses, tyrosin, and 
tryptophane. 
The Biuret test consists in the addition of one drop 
of CuSO, sol. and then KHO 
albumoses, a violet colour indicates unchanged protein. 
Tyrosin is tested for by adding Millon’s reagent to a 
a rose coloration indicates 
