449 G. P. DARNELL-SMITH. 
Agar Plate—Surface colonies slow growing, roundish, white at. 
first, then brownish. 
Bouillon—Clouds with formation of flocculent particles in top 
layers. 
Pigment— Brown in agar and gelatine; soluble in water and 
glycerine; insoluble in absolute alcohol, sulphuric ether, 
chloroform, turpentine, benzine, xylol, benzole, and carbon 
bisulphide. 
Potato slope—Grows readily, producing pale to dark brown 
stain. Reduces nitrates to nitrites. 
Table I—Potato BB 689 (B. solanacearwm ?) 27/11/17. 
No. 1. Incubated 14 days at 38°. 
Morphology etc.—Gram negative, cocci to short rods often in pairs 
Sugars—No acid or gas. 
Milk—Clotted. 
Litmus-milk— Bluish-purple clot. 
Gelatine—No liquefaction (5th day). 
Agar-plate—Dirty white moist circular semi-transparent. 
colonies (3rd day). 
Broth—Flocculent surface growth and deposit. 
Potato—Copious moist growth becoming brownish. 
No. 2 as No. 1, but the blue colour of litmus-milk became dis- 
charged in 14 days. 
Table I1—Tomato BB 98 (B. solanacearum ?) 27/11/17. 
Results at end of 4 weeks. 
Sugars—No acid or gas, 
Milk— Digested. 
Litmus-milk—Indigo blue. 
Gelatine—Liquefied with green fluorescence, dense clouded 
growth at bottom of liquid. 
Broth—Pellicle and deposit. 
Potato—Dirty white to brownish. 
There is therefore strong prima facie evidence that. 
Bact. solanacearum exists in Australian soil. Some ex- 
