BACTERIAL DISEASE OF TOBACCO. 447 
The inoculated plants (white Burley tobacco plants) after 
two months showed a tumescence of the stem and a slight 
yellowing of the vascular tissue. The cell nuclei as in 
tumid diseased plants in the field were frequently much 
enlarged. Check plants stabbed at the same time witha 
sterile needle showed no alteration in growth. 
Cultures were obtained from the inoculated plants at 
the end of two months and the reactions of the organism 
which are given in Table IV leave no room to doubt that 
it is the same organism as that inoculated. It is note- 
worthy that two of the colonies examined gave no acid 
and gas on the sugars, a characteristic of B. solanacearum. 
Table IV.—Organisms recovered from Inoculated Tobacco Plant, 
Pot No. 2. 
No. 2) Q) No. 2 (1) No. 2 (2) No. 2 (3) 
Sugars— 
Glucose acid, gas no change | } 
Mannite | acid, gas i, | 
Dulcite no change % bas 2 (1) as 1 (1) 
Lactose acid, gas (slight) Pos | 
Saccharose} acid, gas es J 
Milk clot, separation,| separation, | as 2 (1) asm i(( 1) 
gas digestion 
Litmus milk 
clot, separation, 
gas decolorised 
almost com-| as 2 (1) 
plete diges- 
clot, separa- 
tion, no gas 
tion, liquid decolorised 
brown 
Gelatine irregular surface) liquefied, as 2 (1) gran-| as 1 (1) 
growth, broken| half inch) ular liquid 
margin, wavy band only 
puncture growth 
no liquefaction 
Agar plate | dirty white, dirty white.| greycircular| large grey, 
rounded,slightly| circular, thin colony} thin colony 
Wavy margin convex, submerged| submerged 
moist 
Broth pellicle, turbid | turbid, film, turbid,| no film, tur- 
deposit deposit bid, deposit 
Remarks.—Results at end of seven days—1 (1) and 2 (8) is the same 
organism that was inoculated; recovered at end of two months. 
