448 
G. P. DARNELL -SMITH. 
A second series of inoculations was carried out, the 
roots and not the stem of the plants being inoculated. 
Colony 1, Plate 1[1(1)] and Colony 1, Plate 2[2(1)] were 
inoculated into roots and recovered after 47 days. 
Details of the reactions of the recovered organisms are 
given in Table V. 
Table V.—Inoculated Tobacco Plants, Second Serves. 
Morphology 
Sugars—Glucose 
Mannite 
Dulcite 
Lactose 
Saccharose 
Milk 
Litmus milk 
Gelatine 
Agar plate 
Broth 
Potato 
No.1 (1) BB 72. From 
Pot 1, root inoculated. 
short rods; gram negative 
acid and gas 
acid and gas 
acid and gas (slight) 
acid, no gas 
' acid, no gas 
tendency toclot, clot and 
separation (6 days) 
slight clot, separation 
slow funnel liquefaction 
dirty white to grey, den- 
dritic, moist (5 days) 
thin, pellicle, turbid,deposit 
fawn, moist spreading 
cocci to short rods, gram 
No. 2 (1) BB 72. From 
Pot 2, root inoculated. 
negative 
acid and gas 
acid and gas 
no change 
_ acid and gas (slight) 
acid and gas 
tendency to clot, clot (6 
days) 
clot, separation 
no liquefaction 
dirty white, circular to 
irregular, moist (3 d.) 
pellicle, turbid, deposit 
white, moist spreading 
In the first series of inoculations (stem inoculations) the 
inoculated plants showed the tumescence of the stem that 
is characteristic of the disease in the early stages. 
In.the second series of inoculations (root inoculations) 
the inoculated plants showed no tumescence up to the time 
at which the organism was re-isolated. We may conclude 
that the organism had lost its virulence, or that root inocu- 
lation does not produce the disease so rapidly as stem 
inoculation. 
Further supplies of diseased plants were obtained and 
plates made from them; the details of the culture reactions 
are given as under:—Plants from Texas, Table VI; from 
Dungowan, Table VII; from Tamworth, Table VIII. 
