452 G. P. DARNELL-SMITH. 
2. Plants that have been attacked by Peronospora 
‘hyoscyamtin the early stages show characteristic symptoms. 
of disease when planted out. 
3. The symptoms are swelling at the base of the stem, 
brown discolouration of the vascular system in the stem 
and leaves, and later cavities in the stem, brittleness of 
the stem and a certain amount of wilting of the leaves. 
| 4. These symptoms are those which have been described 
by HE. Smith, as associated with Granville Wilt in U.S.A., 
(Sumatran slime disease of Tobacco, Japanese Stem Rot, 
etc.) and shown by him to be due to Bact. solanacearum. 
o. A bacterium has always been found associated with 
the disease in New South Wales. 
6. Cultures of the bacteria have been obtained; they 
agree with B. solanacearum in their morphol char- 
acters, but differ culturally. 
7. Plants have been inoculated with pure cultures of the 
bacteria isolated, some symptoms of the disease produced 
and the bacteria reisolated in pure culture. 
8. There are indications that cultural differences obtained 
with different strains of B. solanacearum arise through 
differences in age or treatment of the cultures. 
Literature Cited. 
1. Masser, G.—Diseases of cultivated plants and trees 1910, p.115. 
. Cuinton, G. P.—Oospores of Potato Blight. Report of the 
Connecticut Agricultural Experiment Station, 1909-1910, 
753 —T77A, 
3. SmitH, Erwin—Bacteria in relation to plant diseases, Vol. 11, 
(Os ROW SME 
. fbid., Vol. 1, p. 208, 1914: 
Toid Nola, p, 91, 1911: 
Ibid., Vol. 111, p. 223, 1914. 
. MarsHa.u, C, E.—Microbiology, 2nd edit., 1917, p. 414. 
bo 
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