464 MARGUERITE HENRY, 
Methods Employed. 
When possible the material obtained was examined alive, 
and samples of it were kept alive for some time; this was 
always the case at Kendall, and with the collections made 
at Centennial Park and the University. The specimens 
were drawn with the aid of a camera lucida while still 
alive. Various means of fixing and preserving were tried, 
such as glycerine alcohol, sublimate acetic and Carl’s 
fixative; of these the glycerine alcohol was found to be the 
most satisfactory. Specimens that were unstained and 
had been fixed in glycerine alcohol were placed under a 
bell-jar until the alcohol had evaporated and were then 
mounted in glycerine jelly; these proved to be quite satis- 
factory, and the more delicate crustacea were always 
mounted in this way, as the staining process injured their 
shape and internal structure. The stains used were heema- 
toxylin and borax carmine; the latter proved more suitable 
for those parts that needed closer examination. 
I have to thank Professor 8. J. Johnston for his valuable 
advice and assistance in the preparation of the paper. The 
Cladocera comprised in this paper are— 
Family DaPHNIDz. 
Daphnia carinata King Simocephalus acutirostratus King 
Scapholeberis kingi Sars Ceriodaphnia spinata sp. n. 
Simocephalus elizabethe King Moina tenuicornis Sars 
2 australiensis Dana 
Family LyNCODAPHNID. 
Llyocryptus longiremus Sars Macrothrix spinosa King 
Family LyNnceipa. 
Camptocercus australis Sars Alonella clathratula Sars 
Acroperus avirostris sp. n. Graptoleberis testudinaria Fischer 
fs sinuatus sp Nn. Dunhevedia crassa King 
Alona wallaciana sp. n Pleuroxus reticulatus sp. n. 
»  kendallensis sp. n. Chydorus denticulatus sp. n. 
» longirostris sp. n. 5 clelandi sp. n. 
»  wWhateleggw Sars i, globosus Baird 
»  affinis Leydig 
»  cambourt Richard 
E. ovalis Kurz 
