284 W. L. WATERHOUSE. 



The shoots were kept in the glass cylinders for 24 and 

 36 hours, again with no appreciable difference in the result 

 obtained. After the removal of the glass cylinders, the 

 plants were watered from time to time when the weather 

 was dry. 



After four to five days dark spots became visible on the 

 upper surface of the leaves. As had happened when work- 

 ing with the British rust, sharply defined necrotic areas 

 occurred on some of the inoculated as well as on control 

 shoots. Similar diseased areas were present on leaves of 

 other species of Berheris growing close by. 



Spermagonia appeared on the upper surface of the inocu- 

 lated leaves after 8 to 10 days (Plate XIX, fig. 2). Four 

 to five days later, aecidia were produced on the under 

 surfaces (Plate XIX, fig. 3). Aecidiospores were used to 

 inoculate wheat seedlings. Uredo pustules were produced 

 on the seedling leaves of Hard Federation wheat in 12 

 days. By means of uredospores the rust is being kept in 

 culture for study. 



Aecidiospore measurements were made of 50 spores 

 shaken from an infected barberry leaf. The arithmetic 

 mean was 19*1 /* X 16'7/x and the range from 24*2 /x x 18*6/* 

 to 14*9 p x 14'9/x. Grove (10) gives the measurement as 

 varying from 14 to 26 /^ in diameter. There is therefore 

 no notable difference in the size of the Australian aecidio- 

 spores. 



Sections were cut through infected leaves (Plate XIX, 

 l\gs 4 and 5). A comparison of these slides with others of 

 similar material prepared in England again fails to reveal 

 any differences of note. 



General Discussion. 

 The results obtained show that it is not true that Puc- 

 cinia graminis as it occurs on wheat in Australia has lost 



