18387] — _ Microscopy. 677 
in the adult. The cephalic ganglia give rise to the optic nerves ; 
the rostral ganglia, which occupy a place on the supero-lateral 
face of the supra-cesophageal ganglia, are compared with the 
labial ganglia described by Tichomiroff in Bombyx, while the 
mandibular ganglia, which also enter into the composition of 
the brain, give rise to the sympathetic nerve. 
The speculations which conclude the article, as to the homol- 
ogies of the nervous system in various Metazoa, are not equal to 
the rest of the paper.—/. S. K. 
MICROSCOPY.: 
Method of Staining and Fixing the Elements of Blood.?— 
Recent discoveries af morphological elements in the blood hitherto 
unknown, as well as the newly published facts concerning its Co- 
agulation, have aroused an interest in the subject which calls for 
an acquaintance with the methods with which it is possible to 
follow those results. Accordingly, I would like to describe the 
method employed in this laboratory; for, although it has been 
mentioned by Professor Gaule in his lectures for several years, 
it has not as yet been published. oe 
The methods formerly used were that of examining fresh 
blood and that, perfected by Ehrlich, which consisted in stain- 
ing dried blood. fe 
= Our method consists in a series of manipulations requiring 
only thirty-five minutes for their completion. 
The following is a list of the reagents, together with the 
length of time and the order in which each is to be used: 
Min. 
I. Corrosive sublimate (concentrated solution) . 6 
2. Distilled water . j ; : : . r 
3. Absolute alcohol . ; i . . 5 
4. Distilled water . j 2 . rites ; : 
5. Hematoxylin (14 per cent. alum solution to 
which, for every 100 c.cm. employed, 20 drops 
5 per cent. alcoholic solution have been added) 6 
6. Distilled water . ‘ . . . Hoge 
7. Nigrosin (14 per cent. water solution) . I 
I Dadada o ad a ao a Oe 
9. Eosin (1 gr. eosin dissolved in 60 c.cm. alcohol ; 
140 c.cm. distilled water) . . . . . 2 
10. Alcohol; pe ues 5 
It. Oil of cloves $ : : : . : me 
12. Xylol. 
13. Canada balsam (diluted with xylol until it readily 
flows). To 
As receptacles for these fluids, each person has upon his table 
Edited by C. O. WHITMAN, Milwaukee, Wisconsin. 
? From the Physiological Laboratory at Zurich. 
