1887] ~ Microscopy. 679 
six minutes, is brought again into distilled water for half a minute, 
since our coloring fluids are water solutions. The hematoxylin 
is then dropped upon the slide, and removed again at the end of 
six minutes by resting’ the edge of the slide upon filter-paper, 
and afterwards washing with distilled water for one minute. The 
same process follows with the nigrosin and eosin, the first re- 
maining upon the slide for one minute, the second two minutes. 
From the eosin we bring the preparation directly into alcohol, 
since the eosin is partially an alcohol solution. At the end of 
five minutes the slide is taken out of the alcohol, and, in order 
to be quite sure that there is no water still clinging to the 
preparation, we incline the slide at a slight angle to the rag 
with which we are holding it, and pour a few drops of alcohol 
from the small bottle over it. If upon dropping oil of clovés— 
on the preparation it should be dark upon a dark sleeve or 
other dark background, we may remove the oil of cloves with 
, a few drops of xylol. Having quickly cleaned the slide close 
up to the preparation, we place a drop of Canada balsam upon 
it, which must be allowed to spread out before the cover-slip is 
lowered upon it. 
are also generally homogeneous, though occasionally granulated 
like the nuclei of other cells. 
-Second kind 
Sparely surrounded with protoplasma, colored blue with nigrosin. 
: The form of the cell, according to the position in which we see 
M iS spindle-shaped, with an oval nucleus in which the granules 
PI 
= 
