1895.] Notes on the Reproduction of Plumularian Hydroids. 967 
Under date of April 27th, four days later, I find the follow- 
ing note: 
“To-day I noticed some delicate, thread-like lines adhering 
to the inside of a jar containing living colonies of P. pinnata. 
Upon moving a piece of stone, I found that these lines were 
the long, thread-like processes or continuations of hydrocladia 
noticed several daysago. Uponclose investigation hydranths 
were seen fully expanded arising from these processes attached 
to the glass, and one small colony with the primate branching 
of Plumularia had advanced so far as toshow seven hydranths 
on branches. The original process from the hydrocladium of 
the parent colony has become a creeping stolon attached to 
the glass. It is sending up the new colony on the one hand, 
and giving forth delicate rootlets on the other. A single 
hydranth growing on the stolon a little to the right of the in- 
cipient colony already described, seems to indicate the starting 
of a second colony. Several other stolons (derived in the same 
way from greatly elongated hydrocladia) are giving off little 
colonies. There have been no other plumularians in this jar, 
and the original colonies were without gonangia.” 
These new colonies were kept alive for a week longer, by 
which time their connection with the parent stocks had been 
destroyed by atrophy of the hydrocladial extensions from 
which the new colonies arose, and the daughter colonies had 
attained considerable size and all the characteristic features of 
P. pinnata. 
In another jar a colony showing the hydrocladial extensions 
was purposely placed so that they could reach neither the side 
of the jar nor any other point of support. This did not inter- 
fere with the asexual reproduction, however, as the processes 
became forked at their distal ends, and from these forks arose 
incipient colonies. After a week had elapsed the parent col- 
ony died and the main stem became withered and dropped to 
the bottom of the jar, carrying with it the daughter colonies 
which were then able to attach themselves and proceed with 
their development as would any other colony. 
After a careful search through the literature of the sub- 
ject, I am unable to find any account of this mode of re- 
