20 The Significance of Sex. [ Jan. 
cleus runs like an axis naa all the stems and branches, and is segmented off into 
all the buds and 
Fic. 49, a—A. ibihiblan pancreas cell—Ogata, A. A. P. (Phys. Abth.) 1883.—The 
tissue hardened in warm corrosive sublimate is cut into thin sections and stained suc- 
cessively with hematoxylin, nigrosin, eosin, and safranin. Gaule, the author of this 
method, claims that there are two substances in the cy¢op/asm, one eosinophilous, the 
other nigrosinophil ous. There are also two substances in ager cleoplasm, one stain- 
ing best with Aematoxylin (ordinary chromatin), and the other EUa with safranin. 
The Sai is represented dead-black or heavily saai the safraninophil 
is outlined only; nei eosinophil is marked with parallel lines, the migrostnophi/ by 
crossed lines and 
A. The roar ie imbedded in cytohyaloplasm Sinerat well marked on 
one side. On t e other are the zymogen granules (eosinophi A sparse reticu- 
lum, several si ad and mostly peripheral nucleoli canbe: in one large nu- 
cleolus, the “ plasmosoma” (safraninophil), occupy the nucleus. In å the plasmo- 
soma is migrating from the nucleus muah ae now atrophies. In ¢ the plasmosoma, 
now in the cytoplasm, begins to develop the two constituents of cytoplasm in its in- 
terior. It grows rapidly (ď) to the size of the old nucleus, alongside which it lies. 
In e it has become still larger, and most of it has become transformed into en 
granules and cytoplasm. In the centre of the remainder, f, a chromatin nucleus 
appears, which, later, differentiates in its interior, the plasmosoma and other nucleoli, 
£ a eae so we are back to stage @ again. 
. 50. Nucleus of egg of Colymbetes fuscus (Will, Z. w. Z., xliii.), durin 
soe and yelk formation.—a, reticulated; 4, nucleo eyed ¢, the ae 
growing and enlarging one or more of the nucleoli until all i 
It then buds off large and small cells; the former become siih “aud Sack: 
the latter become follicle-cells. Then sheet after sheet of the nucleus dissolves off 
es, and microsomata of a beaded filament in a reticulum enclosing several nucleoli 
(ih, y , and finally the karyokinetic spindle of the polar globule is formed. 
FIG. 51. aiee of pA of Ascaris megalocephala—b, c, Van Beneden, A. B., iv. ; 
one large sera oars? (the « prothyalosome a, a sereal Rema ranger 505 
the prothyalosome alone takes part in forming the 
ulates with the male pronucleus ; ¢ is the nucleolus of the prothyalsomie hidhi ik tap- 
nified, seen to consist in this stage of two disks, each of four-beaded filaments es the 
and nucleoli. In 6 the reticulum is broken upinto nucleoli. In c these have fused 
tot 
Fic. by. Nucleus of egg of Nephthys scolopendroides—Carnoy, p. 237. 
54, 2-6. Nucleus of egg of Fie/d-Mouse—Rauber. M. J., viii. 
Fic. 35s a—b. Nucleus of egg of Perch—Rauber. M. J., viii—In æ the micro- 
somata are e superficial and their processes s a reticulum ; å is an optic section. 
oat: oo Prate III. | 
Fic. 56, a-f. Nucleus of f Arion duri i i a yak formation, Pin- 
ede ws E we see a nucleolus and 1 microsomata and a 
ve SS E E PET PERE ENRE a sparse reticulum, but the 
cros 1 nucleolus, while the old nucle- 
