1887] Botany. 179 
not affected by ether, and stains from yellowish brown to dark 
blue with Hanstein’s aniline violet. The cells surrounding 
these contain much starch and protoplasm, with usually a 
nucleus. 
Resin passages are also found in the phlceum and outer cortex, 
which do not run parallel to the long axis of the stem. Some 
were found running radially from towards the centre. At one 
end of some of these radially directed passages they hend at, or 
nearly at, a right angle, and pass up the stem. The great major- 
of growth in the spring. This material was preserved in alco- 
hol and used as desired. Schultze’s solution and Hanstein’s 
aniline violet gave the best satisfaction as staining agents: 
Schultze’s olution for differentiation of tissues, the aniline 
violet for staining nuclei. To clarify the sections, after cutting 
placed them in sulphuric ether for some time, which removed 
the resinous substances, then, if staining with the violet, I 
removed them to a reduced solution of this, and after overstain- 
ing removed the excess with alcohol by placing the sections in 
it for a short time, and afterwards cleared with clove oil and 
mounted in dammar. . 
