120 Germ of the Southern Cattle Plague. 
specimens of the same material, or take any other slides of an 
object of the same size and form, and observe such for about half an 
hour, thus preparing the eye to see what you want to see in the liv- 
ing developing organism. Unless this is done, some very essential 
points will be surely missed, and some preventable error fallen into. 
With anything less than a power of 800 diameters no one should 
attempt to study these organisms, and then only when aided by the 
best of Abbe condensers and oil immersion lenses. 
We left our studies with the mature object proliferated into its 
first distinct stage of vegetative di erentiation. We had two coc- 
coid objects before us, that is, two round objects, their diameters 
being the same in any direction. If colored, they color throughout, 
that is, diffusely. 
Were these objects to remain in this condition, they would be, 
indeed, Micrococct. They do not, however. They almost imme- 
diately begin to increase in a longitudinal direction, but in this con- 
dition they still stain diffusely. 
In my first description of the swine-plague germ, I said that the 
next biological phenomenon was the appearance of a delicate white 
line, separating this ovoid object into two halves. The above, while 
not exactly an erroneous description, is certainly anticipated by 
another phenomenon in the evolutional development of this coccoid, 
- diffusely coloring object, into the mature form of any of this class 
- of germs. That this white non-coloring substance is a secretion of the 
two poles, or coccoid ends, of these “ belted” germs, as well as that 
it has a different chemical composition, is beyond all question. 
The phenomenon above spoken of, as anticipating the formation 
of the segmenting white line which separates the two darker por- 
tions of these organisms is: that this white substance first appears in 
the centre of the body of the dense, dark ovoid object as the minutest 
of white specks, which gradually increases in size and quantity, and 
extends across the entire object ; the white line, being at first broader 
in the middle, but gradually widening until it completely and clearly 
separates the two pole (coccoid) ends, and the mature object is again 
presented to our view (Fig. 6). 
We have thus described the normal, or general, cycle of devel- 
opment of the micro-etiological organism of the American, English 
and German Swine Plagues, the American Southern Cattle Plague, 
Hen Cholera, the German “ Wild-Seuche” (of deer, swine and cat- 
tle) and Rabbit Septicemia, all of which diseases are caused by a 
