126 Germ of the Southern Cattle Plague. 
steer, and material from a ground squirrel. In making the gelatine 
tube, I simply inoculated from one agar agar tube on two gelatine 
tubes, with no other precaution than a macroscopic comparison of the 
growth with those in the other agar agar tubes. I could see no change 
in the appearance of the growth of the tubes I used. J should 
have made, and every one should always make a few cover-glass 
specimens for the microscopic test in all such eases. (In the case of 
these germs, it would be futile, however.) After the cultures in the 
gelatine had become fluid, I then inoculated the entire agar cul- 
tivations (twenty) upon gelatine, and carefully numbered each tube 
with a corresponding number, so as to control the number. 
This time I was not at all surprised where in nineteen of the beef- 
infusion gelatine tubes no fluidification had taken place, the same 
occurring in the one as before and from the same agar tube. It is now 
February 6th, and the tubes remain exactly as they were on the 8th of 
October. 
Hence, the germs of the Southern Cattle Plague, like those of the 
American Swine Plague, and other diseases of the same group, that 
are caused by the belted oval germs do not cause fluidification of 
gelatine media. 
I next inoculated twenty pieces of sterilized potatoes (and for 
comparison’s sake twenty others from cultivations of the Swine- 
Plague germ), and here I found no change in the appearance of the 
growths from those previously described. From the twenty potatoe 
culture of the Southern Cattle Plague germ I again inoculated 
twenty gelatine tubes. Nineteen remained solid ; one became fluid. 
As the potato culture from the tubes which caused the gelatine t0 
become fluid did not show any variation in the color of the gro 
upon agar agar from the others, I resorted to plate cultivations a5 
well as the microscope to solve the riddle. : 
This one tube contained a small number of the most contempt- 
ibly small micrococci, yet enough to have got me into a serious 
error. They required 2,000-diameter amplification to see them 
distinctly, and, as I have said, Micrococci constitute a normal mor- — 
phos in the development of this class of germs, their presence 
would have excited no suspicions had I subjected the original cul- 
ture to a microscopic examination. Still it should be done in eve f 
case, so as to keep up a good rule. a 
They were separated with ease on plates. Inoculation up — 
Gophers with the mixed culture gave fatal results, but no coc? — 
