1894.] Embryology. 531 .; 
divides ihto two chromatin spherules, then, by successive divisions, 
there is formed a great number of spherules which remain inclosed in 
a clear areole. This clear areole recalls the hyaline centre of attrac- 
tion when that has received the half plate for the formation of a vesi- 
cular nucleus.’ ; . 
—F. L. WASHBURN. 
1 The following few not taining to the fixi d staining of freshly laid eggs 
££ D o 
Eggs placed for 5 minutes in Fol 99 (1 % chromic 25 vol, 2 % acetic 50 vol, 
H,O 25 vol) then shelled in water, vitellus in same solution for 5 minutes, H,O 
10 min., and 35 % and 50 % alcohol 5 minutes each, 70 % 30 min. and 90 % ad. 
lib. gave good results, taking picrocarminate of lithium very well if left long 
enough in stain. They also took borax carmine very well after the above treat- 
ment. x 
Both of these stains did well after the eggs were immersed in chromic + % 10 
min., then shelled in large quantity of water, then vitellus in chromic } % 4 min., 
and H,O and grades of alcohol as above. 
Whole egg in osmic acid 1 % 5 min., followed by Merkel’s fluid 4 hrs. ; shell, 
then water and grades of alcohol 2 min. each to 70 % for permanent preservation 
were quite satisfactory. It gave good results as to nuclei when eggs were left in 
picrocarminate of lithium for 48 hrs. 
